Our products and services include:
- MHCII affinity and stability assays.
- Customized biotinylated MHCII/peptide tetramers.
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MHC II affinity and stability assays
Computational methods to identify MHC binding peptides exist, they do however tend to be imprecise and over-predictive.
Immunitrack offers state of the art peptide MHC affinity measurements by very sensitive assays. The assays use nanomolar concentration of MHC II and measure the direct binding of peptide to MHC II. The assays have been validated in several NIH funded projects and peer reviewed articles and account for more than 100.000 Kd measurements in the Immune Epitope Database (IEDB).
For MHC I it was shown that stability is a better indicator of immunogenicity than affinity, preliminary results from Immunitrack supports this hypothesis for MHC II. Our stability assay measures the decay of MHC II complexes at physiological temperature enabling precise half-life determinations. All assays are amendable for HTS screening and does not require any modifications of peptide.
Affinity vs. stability EBV
Affinity vs. stability CMV
Biotinylated MHC II complexes
Our biotinylated MHC II complexes have been used as tetramer reagents in several publications. The previous challenges of producing MHC II molecules of a sufficient quality have hampered the disseminated use of MHC II tetramers. By using a tag on the peptide our production method ensures a 100 % peptide loading of complexes.
Immunitrack is continuously expanding its MHC II repertoire and are currently able to deliver assays or complexes on the following alleles:
MHCII alleles not present on the list may be synthesized and produced upon request.
Publications where the MHC II molecules were used
A carbon nanotube-polymer composite for T-cell therapy.
Nat Nanotechnol. 2014 Aug;9(8):639-47
Identification and HLA-tetramer-validation of human CD4+ and CD8+ T cell responses against HCMV proteins IE1 and IE2.
PLoS One. 2014 Apr 23;9(4):e94892.
Adsorption of multimeric T cell antigens on carbon nanotubes: effect on protein structure and antigen-specific T cell stimulation.
Small. 2013 Mar 11;9(5):666-72.
MHC class II tetramers made from isolated recombinantαandβchains refolded with affinity-tagged peptides.
PLoS One. 2013 Sep 2;8(9):e73648.
Chaperone-assisted thermostability engineering of a soluble T cell receptor using phage display.
Sci Rep. 2013;3:1162.
Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides.
Immunology. 2011 Apr;132(4):482-91.
NetMHCIIpan-2.0 - Improved pan-specific HLA-DR predictions using a novel concurrent alignment and weight optimization training procedure.
Immunome Res. 2010 Nov 13;6:9
HLA class I binding 9mer peptides from influenza A virus induce CD4 T cell responses.
PLoS One. 2010 May 7;5(5):e10533.
Functional recombinant MHC class II molecules and high-throughput peptide-binding assays.
Immunome Res. 2009 May 5;5:2.
Quantitative predictions of peptide binding to any HLA-DR molecule of known sequence: NetMHCIIpan.
PLoS Comput Biol. 2008 Jul 4;4(7):e1000107.