MAJOR HISTOCOMPABILITY COMPLEX CLASS I

 

Assays and molecules

 

Our products and services include:

- MHCI affinity and stability assays.

- Customized biotinylated MHCI/peptide complexes.

For quotes and orders please download form and send it to us: Form for download


 

 

MHC I affinity and stability assays

Computational methods to identify MHC binding peptides exist, they do however tend to be imprecise and over-predictive. Immunitrack offers state of the art peptide MHC affinity measurements by very sensitive assays. The assays use nanomolar concentration of MHC I and measure the direct binding of peptide to MHC . The assays have been validated in several NIH funded projects and peer reviewed articles and account for more than 100.000 Kd measurements in the Immune Epitope Database (IEDB). It was shown that stability is a better indicator of immunogenicity than affinity (Eur J Immunol. 2012 Jun;42(6):1405-16). Our stability assay measures the decay of MHC I complexes at physiological temperature enabling precise half-life determinations. All assays are amendable for HTS screening and do not require any modifications of peptide.
                               

Affinity measurements of 4 tumor antigen peptides, MART-1 WT (Kd~23nM) binds nearly 10 times weaker than the A27L mutant (Kd~2 nM). Stability assessment of the two MART-1 variants shows an increased stability of the A27L variant compared to WT (Half-lives: 8 and 2 Hrs respectively).


 

 

Biotinylated MHC I complexes

Our biotinylated MHC I complexes have been used as tetramer reagents as well as for the stimulation of CD8 T cells in several publications. Please contact us for further information or a quote.

                                        

B*0801 Tetramer staining of two CD8 T cells specific for peptides derived from HCMV Protein IE1. Adapted from: PlosOne April 2014, Volume 9,Issue 4,e94892 Identification and HLA-Tetramer-Validation of Human CD4+ and CD8+ T Cell Responses against HCMV Proteins IE1 and IE2


 

Alleles available

List of currently available MHC

Human Murine
HLA-A HLA-B HLA-C Kb
A*01:01 B*07:02 C*04:01 Db
A*02:01 B*08:01 C*07:01 Kd (available end Q4 2016)
A*03:01 B*14:02 C*07:02 Kk (available end Q4 2016)
A*11:01 B*15:01 Dd (available end Q4 2016)
A*24:02 B*18:01 Dk (available end Q4 2016)
A*25:01 B*35:01
A*31:01 B*42:02
A*74:01 B*44:03
B*52:01

MHCI alleles not present on the list may be synthesized and produced upon request. 


 

Publications where the MHCI were used

1.
Mismatch-repair deficiency predicts response of solid tumors to PD-1 blockade
Science, 2017 Jul 28;357(6349):409-413
PMID: 28596308
2.
Configuration-dependent presentation of multivalent IL-15:IL-15Rα enhances the antigen-specific T cell response and anti-tumor immunity.
J Biol Chem. 2015 Dec 30. pii: jbc.M115.695304.
PMID: 26719339
3.
A carbon nanotube-polymer composite for T-cell therapy.
Nat Nanotechnol. 2014 Aug;9(8):639-47.
PMID: 25086604
4.
Identification and HLA-tetramer-validation of human CD4+ and CD8+ T cell responses against HCMV proteins IE1 and IE2.
PLoS One. 2014 Apr 23;9(4):e94892. doi: 10.1371/journal.pone.0094892. eCollection 2014.
PMID: 24760079
5.
Adsorption of multimeric T cell antigens on carbon nanotubes: effect on protein structure and antigen-specific T cell stimulation.
Small. 2013 Mar 11;9(5):666-72. doi: 10.1002/smll.201201684. Epub 2012 Oct 23.
PMID: 23090793
6.
Wildtype p53-specific antibody and T-cell responses in cancer patients.
J Immunother. 2011 Nov-Dec;34(9):629-40. doi: 10.1097/CJI.0b013e3182281381.
PMID: 21989411
7.
Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides.
Immunology. 2011 Apr;132(4):482-91. doi: 10.1111/j.1365-2567.2010.03383.x. Epub 2011 Feb 7.
PMID: 21294723
8.
HLA class I binding 9mer peptides from influenza A virus induce CD4 T cell responses.
PLoS One. 2010 May 7;5(5):e10533. doi: 10.1371/journal.pone.0010533.
PMID: 20479886
9.
Peptide binding to HLA class I molecules: homogenous, high-throughput screening, and affinity assays.
J Biomol Screen. 2009 Feb;14(2):173-80. doi: 10.1177/1087057108329453. Epub 2009 Feb 4.
PMID: 19196700
10.
One-pot, mix-and-read peptide-MHC tetramers.
PLoS One. 2008 Feb 27;3(2):e1678. doi: 10.1371/journal.pone.0001678.
PMID: 18301755
11.
NetMHCpan, a method for quantitative predictions of peptide binding to any HLA-A and -B locus protein of known sequence.
PLoS One. 2007 Aug 29;2(8):e796.
PMID: 17726526
12.
Definition of supertypes for HLA molecules using clustering of specificity matrices.
Immunogenetics. 2004 Mar;55(12):797-810. Epub 2004 Feb 13.
PMID: 14963618

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