Exploiting FcRn mediated rescue has emerged as a generalizable approach to improving the pharmacokinetics of therapeutics. The ability to measure FcRn interactions is a prerequisite for the study and eventual manipulation of FcRn mediated rescue. Immunitrack offer FcRn from a variety of species (human, mouse, rat , pig, dog, guinea pig and cow) all are produced with the corresponding beta 2 microglobulin. For expression purposes unpaired cysteines are mutated to serines, this does not affect the ligand binding characteristics of the FcRn´s. The molecules are 100 % active and can be supplied with or without an enzymatically biotinylated C terminus.
The molecules have been tested successfully on a variety of platforms (AlphaScreen, Octet and Biacore), please see below. An application note on the Biacore platform was developed in a collaboration with GE healthcare, it can be downloaded here: FcRn Immunitrack GE collaboration
For quotes and order please download form and send it to us: Form for download
New publication where, our human FcRn was used: Neonatal Fc Receptor Binding Tolerance toward the Covalent Conjugation of Payloads to Cysteine 34 of Human Albumin Variants
Customer testimonial from Novozymes
Novozymes Biopharma uses the FcRn from Immunitrack to develop the albumin based half-life extension platform Veltis®. We have tested a number of commercially available FcRn’s in our screening assay developed for identification of albumin variants with improved affinity for FcRn receptor and found that the biotinylated FcRn’s from Immunitrack deliver superior performance. These biotinylated FcRn’s enable a robust immobilization to Streptavidin-biosensors (Octet Red96 from Pall ForteBio) and deliver very consistent and robust data.
Products and prices
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Performance of biotinylated FcRn on different assay platforms
AlphaScreen(TM) , Perkin Elmer
Octet Platform, Fortebio
Biacore, GE heathcare Life Sciences
Examples showing binding profiles for different types of antibodies to human, macaque and mouse FcRn. Binding behavior towards human and macaque FcRn was very similar. All antibodies showed highest binding towards mouse FcRn. Binding specificities for all antibodies were in agreement with published data (*Abdiche, Y. N. et al. 2014, mAbs, 7, 331-338). Biotin CAP Kit (GE Healthcare) was used with FcRn capture levels 90-100 RU and antibody concentration series: 25, 74, 222, 666 and 2000 nM. Running buffer: 20 mM phosphate, 150 mM NaCl, 0.05 % Surfactant P20, pH 6.0
List of publications where the FcRn were used:
Materno-fetal transfer of preproinsulin through the neonatal Fc receptor prevents autoimmune diabetes.
Diabetes. 2015 Apr 27.
Regulation of immune responses to protein therapeutics by transplacental induction of T cell tolerance.
Sci Transl Med. 2015 Feb 18;7(275)
Stabilisation of the Fc fragment of human IgG1 by engineered intradomain disulfide bonds.
PLoS One. 2012;7(1):e30083.
Ligand binding and antigenic properties of a human neon.
FEBS J. 2008 Aug;275(16):4097-110.
A strategy for batal Fc receptor with mutation of two unpaired cysteine residues. Bacterial production of a soluble functional human neonatal Fc receptor.
J Immunol Methods. 2008 Feb 29;331(1-2):39-49.